Fasciola gigantica cathepsin L proteinase-based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F08%3A00307258" target="_blank" >RIV/61388963:_____/08:00307258 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Fasciola gigantica cathepsin L proteinase-based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis
Original language description
We herein report on immunodiagnosis of fasciolosis, and significant protection of sheep against challenge infection with Fasciola gigantica following immunization with peptides based on the H-Asp110-Lys-Ile-Asp-Trp-Arg-Glu-Ser-Gly-Tyr-Val-Thr-Glu-Val123-OH (Fas14p) sequence of F. gigantica cathepsin L-cysteine proteinase. This sequence was synthesized as 1) N .alpha. acetylated (FasAc14p), 2) bearing at the aminoterminus an N .alpha.-acetylated cystein (FasAcCys14p), and 3) conjugated in four copies tosequential oligopeptide carrier Ac-[Lys-Aib-Gly]4-OH (Ac-SOC4) through an amide bond (Ac-[Lys(Fas14p)-Aib-Gly]4-OH). The peptides were synthesized manually by SPPS on a Wang resin using the Fmoc/tBu strategy. All compounds were tested as immunodiagnostics for sheep fasciolosis. The data together indicated that the peptides might be of considerable use for discriminating between early and late, and low and high burden, sheep infection with F. gigantica.
Czech name
Synthetické peptidy odvozené od Fasciola gigantica cathepsin L proteinasy a jejich využití pro imunodiagnostiku a prevenci fasciolosy u ovcí
Czech description
Práce popisuje imunodiagnostiku fasciolosy a signifikantní protekci ovcí vůči infekci parazitem Fasciola gigantica po imunizaci peptidy založenými na sekvenci H-Asp110-Lys-Ile-Asp-Trp-Arg-Glu-Ser-Gly-Tyr-Val-Thr-Glu-Val123-OH (Fas14p) z F. gigantica cathepsin L-cysteine proteinasy. Tato sekvence byla syntetizována jako 1) N alfa acetylovaná (FasAc14p), 2) nesoucí na aminokonci N alfa-acetylovaný cystein (FasAcCys14p), a 3) konjugovaná ve čtyřech kopiích na sekvenční oligopeptidový nosič Ac-[Lys-Aib-Gly]4-OH (Ac-SOC4) pomocí amidové vazby (Ac-[Lys(Fas14p)-Aib-Gly]4-OH). Peptidy byly syntetizovány manuálně metodou na pevné fázi (SPPS) na Wangově pryskyřici pomocí Fmoc/tBu strategie. Všechny látky byly testovány jako imunodiagnostika ovčí fasciolosy. Získané výsledky ukazují, že peptidy mohou mít značný význam pro rozlišení mezi časnou a pozdní, a nízkou a vysokou zátěží u infekce ovcí s F. gigantica.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biopolymers
ISSN
0006-3525
e-ISSN
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Volume of the periodical
90
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
349-357
UT code for WoS article
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EID of the result in the Scopus database
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