Extraction and separation of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species by CZE

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F09%3A00336576" target="_blank" >RIV/61388963:_____/09:00336576 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Extraction and separation of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species by CZE

Original language description

A new method for extraction and capillary electrophoretic (CE) separation and characterization of water-soluble proteins (albumin fraction) from Bacillus thuringiensis-transgenic and non-transgenic maize varieties was developed. The best CE separation ofextracted proteins was achieved in isoelectric background electrolyte (200mM iminodiacetic acid, pH 2.26). Well resolved components of protein extracts were characterized by effective electrophoretic mobilities and corrected peak areas. Significant qualitative and quantitative differences in CE-UV profiling were found, which could potentially be used to distinguish the B. thuringiensis variety from the wild maize species.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F08%2F1428" target="_blank" >GA203/08/1428: Capillary electrophoresis with multidimensinal detection and its application to separation and characterization of biopolymers</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

21

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

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UT code for WoS article

000273993400026

EID of the result in the Scopus database

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