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Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F10%3A00353703" target="_blank" >RIV/61388963:_____/10:00353703 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA

  • Original language description

    A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR).

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

    CC - Organic chemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2010

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    Current Protocols in Chemical Biology

  • ISBN

    9780470559277

  • Number of pages of the result

    14

  • Pages from-to

  • Number of pages of the book

    550

  • Publisher name

    Wiley Interscience

  • Place of publication

    New York

  • UT code for WoS chapter