2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00360024" target="_blank" >RIV/61388963:_____/11:00360024 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.4161/epi.6.6.16215" target="_blank" >http://dx.doi.org/10.4161/epi.6.6.16215</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4161/epi.6.6.16215" target="_blank" >10.4161/epi.6.6.16215</a>
Alternative languages
Result language
angličtina
Original language name
2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential
Original language description
A comparative study of hypomethylating activities of a series of 5-azacytidine nucleosides: 5-azacytidine, 2´-deoxy-5-azacytidine and its alpha-anomer, 5,6-dihydro-5-azacytidine, 2´-deoxy-5,6-dihydro-5-azacytidine and its ?-anomer, and of a 2-pyrimidoneribonucleoside (zebularine) was conducted. Methylation-specific PCR was employed to detect the efficiency of individual agents on cyclin-dependent kinase inhibitor 2B and thrombospondin-1 hypermethylated gene loci. Overall changes in DNA methylation level were quantified by direct estimation of 5-methyl-2´-deoxycytidine 5´-monophosphate by HPLC using digested genomic DNA. Flow cytometric analysis of cell cycle progression and apoptotic markers was used to determine cytotoxicity of the compounds. mRNA expression was measured using qRT-PCR. 2´-Deoxy-5,6-dihydro-5-azacytidine was found to be less cytotoxic and more stable than 2´-deoxy-5-azacytidine at the doses that induce comparable DNA hypomethylation and gene reactivation.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1M0508" target="_blank" >1M0508: New Antivirals and Antineoplastics</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Epigenetics
ISSN
1559-2294
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
769-776
UT code for WoS article
000291148100013
EID of the result in the Scopus database
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