Fluorescent castasterone reveals BRI1 signaling from the plasma membrane
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00378975" target="_blank" >RIV/61388963:_____/12:00378975 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/12:33142781 RIV/61389030:_____/12:00378975
Result on the web
<a href="http://dx.doi.org/10.1038/NCHEMBIO.958" target="_blank" >http://dx.doi.org/10.1038/NCHEMBIO.958</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/NCHEMBIO.958" target="_blank" >10.1038/NCHEMBIO.958</a>
Alternative languages
Result language
angličtina
Original language name
Fluorescent castasterone reveals BRI1 signaling from the plasma membrane
Original language description
Receptor-mediated endocytosis is an integral part of signal transduction as it mediates signal attenuation and provides spatial and temporal dimensions to signaling events. One of the best-studied leucine-rich repeat receptor-like kinases in plants, BRASSINOSTEROID INSENSITIVE 1 (BRI1), perceives its ligand, the brassinosteroid (BR) hormone, at the cell surface and is constitutively endocytosed. However, the importance of endocytosis for BR signaling remains unclear. Here we developed a bioactive, fluorescent BR analog, Alexa Fluor 647-castasterone (AFCS), and visualized the endocytosis of BRI1-AFCS complexes in living Arabidopsis thaliana cells. Impairment of endocytosis dependent on clathrin and the guanine nucleotide exchange factor for ARF GTPases(ARF-GEF) GNOM enhanced BR signaling by retaining active BRI1-ligand complexes at the plasma membrane. Increasing the trans-Golgi network/early endosome pool of BRI1-BR complexes did not affect BR signaling. Our findings provide what is t
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Chemical Biology
ISSN
1552-4450
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
583-589
UT code for WoS article
000304256300015
EID of the result in the Scopus database
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