Identification of the double-bond position in fatty acid methyl esters by liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00380954" target="_blank" >RIV/61388963:_____/12:00380954 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2012.04.055" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2012.04.055</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2012.04.055" target="_blank" >10.1016/j.chroma.2012.04.055</a>

Result language
angličtina
Original language name
Identification of the double-bond position in fatty acid methyl esters by liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry
Original language description
Fatty acid methyl esters (FAMEs) were analysed by reversed-phase HPLC coupled with atmospheric pressure chemical ionisation (APCI) mass spectrometry. The chromatographic separations of the FAMEs were optimised using acetonitrile or binary acetonitrile gradients and C18 or C30 columns. The gas-phase reactions of acetonitrile and unsaturated FAMEs in the APCI source provided [M+C3H5N]+ adducts. When fragmented, these adducts yielded diagnostic ions, allowing the unambiguous localisation of double bonds. The formation and fragmentation of the acetonitrile-related adduct was utilised for the structural characterisation of the FAMEs separated by HPLC. The APCI-MS detection of FAMEs encompassed a full-spectrum scan (providing information on the number of carbons and double bonds) and a data-dependent MS/MS scan of the [M+C3H5N]+ ions (the position of the double bonds). The utility of this approach was demonstrated using a mixture of FAMEs from blackcurrant-seed oil. All the unsaturated fatty
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Project
<a href="/en/project/GA203%2F09%2F0139" target="_blank" >GA203/09/0139: Complex lipidomic characterization of plant and animal tissues</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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