Effect of Enamel Matrix Derivative and of Proline-Rich Synthetic Peptides on the Differentiation of Human Mesenchymal Stem Cells Toward the Osteogenic Lineage

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00381034" target="_blank" >RIV/61388963:_____/12:00381034 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1089/ten.tea.2011.0404" target="_blank" >http://dx.doi.org/10.1089/ten.tea.2011.0404</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/ten.tea.2011.0404" target="_blank" >10.1089/ten.tea.2011.0404</a>

Result language

angličtina

Original language name

Effect of Enamel Matrix Derivative and of Proline-Rich Synthetic Peptides on the Differentiation of Human Mesenchymal Stem Cells Toward the Osteogenic Lineage

Original language description

With the aim of discovering new molecules for induction of bone formation and biomineralization, combination of bioinformatics and simulation methods were used to design the structure of artificial peptides based on proline-rich domains of enamel matrixproteins. In this study, the effect of such peptides on the differentiation toward the osteogenic lineage of human umbilical cord mesenchymal stem cells (hUCMSCs) was evaluated with or without osteogenic supplements (hydrocortisone, beta-glycerol phosphate, and ascorbic acid) and compared to the effect of the commercially available enamel matrix derivative (EMD). It was hypothesized that the differentiation toward the osteogenic lineage of hUCMSCs would be promoted by the treatment with the synthetic peptides when combined with differentiation media, or it could even be directed exclusively by the synthetic peptides. Osteoinductivity was assessed by cell proliferation, bone morphogenetic protein-2 secretion, and gene expression of osteo

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Tissue Engineering

ISSN

1937-3341

e-ISSN

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Volume of the periodical

18

Issue of the periodical within the volume

11/12

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

1253-1263

UT code for WoS article

000304779400015

EID of the result in the Scopus database

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