Most Anti-BrdU Antibodies React with 2-Deoxy-5-Ethynyluridine ? The Method for the Effective Suppression of This Cross-Reactivity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00386136" target="_blank" >RIV/61388963:_____/12:00386136 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/12:00386136 RIV/68378050:_____/12:00386136
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pone.0051679" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0051679</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0051679" target="_blank" >10.1371/journal.pone.0051679</a>
Alternative languages
Result language
angličtina
Original language name
Most Anti-BrdU Antibodies React with 2-Deoxy-5-Ethynyluridine ? The Method for the Effective Suppression of This Cross-Reactivity
Original language description
5-Bromo-2´-deoxyuridine (BrdU) and 2´-deoxy-5-ethynyluridine (EdU) are widely used as markers of replicated DNA. While BrdU is detected by means of antibodies, the click reaction typically with fluorescent azido-dyes is used for EdU localisation. We haveperformed an analysis of ten samples of antibodies against BrdU with respect to their reactivity with EdU. Except for one sample all the others evinced reactivity with EdU. A high level of EdU persists in nuclear DNA even after the reaction of EdU withfluorescent azido-dyes if the common concentration of dye is used. Although a ten-time increase of azido-dye concentration resulted in a decrease of the signal provided by anti-BrdU antibodies, it also resulted in the substantial increase of the non-specific signal. We have shown that this unwanted reactivity is effectively suppressed by nonfluorescent azido molecules. We tested two protocols of BrdU revelation in experiments of the simultaneous localisation of BrdU and EdU with the supp
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
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UT code for WoS article
000312483300023
EID of the result in the Scopus database
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