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Most Anti-BrdU Antibodies React with 2-Deoxy-5-Ethynyluridine ? The Method for the Effective Suppression of This Cross-Reactivity

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00386136" target="_blank" >RIV/61388963:_____/12:00386136 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081707:_____/12:00386136 RIV/68378050:_____/12:00386136

  • Result on the web

    <a href="http://dx.doi.org/10.1371/journal.pone.0051679" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0051679</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1371/journal.pone.0051679" target="_blank" >10.1371/journal.pone.0051679</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Most Anti-BrdU Antibodies React with 2-Deoxy-5-Ethynyluridine ? The Method for the Effective Suppression of This Cross-Reactivity

  • Original language description

    5-Bromo-2´-deoxyuridine (BrdU) and 2´-deoxy-5-ethynyluridine (EdU) are widely used as markers of replicated DNA. While BrdU is detected by means of antibodies, the click reaction typically with fluorescent azido-dyes is used for EdU localisation. We haveperformed an analysis of ten samples of antibodies against BrdU with respect to their reactivity with EdU. Except for one sample all the others evinced reactivity with EdU. A high level of EdU persists in nuclear DNA even after the reaction of EdU withfluorescent azido-dyes if the common concentration of dye is used. Although a ten-time increase of azido-dye concentration resulted in a decrease of the signal provided by anti-BrdU antibodies, it also resulted in the substantial increase of the non-specific signal. We have shown that this unwanted reactivity is effectively suppressed by nonfluorescent azido molecules. We tested two protocols of BrdU revelation in experiments of the simultaneous localisation of BrdU and EdU with the supp

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CC - Organic chemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    PLoS ONE

  • ISSN

    1932-6203

  • e-ISSN

  • Volume of the periodical

    7

  • Issue of the periodical within the volume

    12

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

  • UT code for WoS article

    000312483300023

  • EID of the result in the Scopus database