Serine protease from midgut of Bombus terrestris males
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00391467" target="_blank" >RIV/61388963:_____/13:00391467 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/13:00391467
Result on the web
<a href="http://dx.doi.org/10.1002/arch.21075" target="_blank" >http://dx.doi.org/10.1002/arch.21075</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/arch.21075" target="_blank" >10.1002/arch.21075</a>
Alternative languages
Result language
angličtina
Original language name
Serine protease from midgut of Bombus terrestris males
Original language description
A serine protease was isolated from midguts of the bumblebee male Bombus terrestris by a combination of precipitation procedures with column chromatography. The purified enzyme exhibited two bands with molecular masses of 25 and 26 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These bands showed a proteolytic activity in zymography assay. Midgut enzymes showed optimum proteolytic activity at pH 9 and 35 degrees C using N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenyl-alanine 4-nitroanilide as a substrate. The Michaelis constant (Km) and maximum reaction rate (Vmax) were 0.55 +/- 0.042 mM and 0.714 +/- 0.056 mol p-nitroalanine produced min1 mg protein1, respectively. Inhibition was affected by trypsin inhibitor, but not by phenylmethylsulfonyl fluoride and N-tosyl-L-phenylalanine chloromethyl ketone, which indicated the trypsin-like but not chymotrypsin-like specificity. The identity of the serine protease was confirmed by nanoliquid-tandem mass spectrome
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Insect Biochemistry and Physiology
ISSN
0739-4462
e-ISSN
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Volume of the periodical
82
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
117-128
UT code for WoS article
000315102100002
EID of the result in the Scopus database
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