Quantification of homocysteine-related metabolites and the role of betaine-homocysteine S-methyltransferase in HepG2 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00391512" target="_blank" >RIV/61388963:_____/13:00391512 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/bmc.2755" target="_blank" >http://dx.doi.org/10.1002/bmc.2755</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/bmc.2755" target="_blank" >10.1002/bmc.2755</a>

Result language

angličtina

Original language name

Quantification of homocysteine-related metabolites and the role of betaine-homocysteine S-methyltransferase in HepG2 cells

Original language description

We optimized and validated a rapid and sensitive liquid chromatographytandem mass spectrometry (LC-MS/MS) method for the quantification of six metabolites of homocysteine metabolism: homocysteine, methionine, cysteine, S-adenosylmethionine, S-adenosylhomocysteine and betaine. The detection limits for these metabolites were in the nanomolar range, and the intra- and inter-day precisions were lower than 20% of the relative standard deviations. The method was specifically designed for the determination ofthe intracellular concentrations of the metabolites in cultured cells. To study the role of betainehomocysteine S-methyltransferase (BHMT), HepG2 cells and HepG2 cells that were stably transfected with BHMT (BHMTHepG2) were treated with homocysteine or with a specific inhibitor of BHMT, and metabolite levels were subsequently measured. Severely compromised methyl group metabolism in the HepG2 cells, which is typical of cancer-derived cells, prevented clear evaluation of the changes cause

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GAP207%2F10%2F1277" target="_blank" >GAP207/10/1277: New inhibitors for human methyltransferases BHMT and BHMT-2 to study their physiological functions</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Chromatography

ISSN

0269-3879

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

111-121

UT code for WoS article

000312306300015

EID of the result in the Scopus database

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