Cation-Specific Effects on Enzymatic Catalysis Driven by Interactions at the Tunnel Mouth

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00394029" target="_blank" >RIV/61388963:_____/13:00394029 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/13:00066616 RIV/00159816:_____/13:00060623

Result on the web

<a href="http://dx.doi.org/10.1021/jp401506v" target="_blank" >http://dx.doi.org/10.1021/jp401506v</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/jp401506v" target="_blank" >10.1021/jp401506v</a>

Result language

angličtina

Original language name

Cation-Specific Effects on Enzymatic Catalysis Driven by Interactions at the Tunnel Mouth

Original language description

Cationic specificity which follows the Hofmeister series has, been established for the catalytic efficiency of haloalkane dehalogenase LinB by a combination of molecular dynamics simulations and enzyme kinetic experiments. Simulations provided a detailedmolecular picture of cation interactions with negatively charged residues on the protein surface, particularly at the tunnel mouth leading to the enzyme active site. On the basis of the binding affinities, cations were ordered as Na+ > K+ > Rb+ > Cs+. In agreement with this result, a steady-state kinetic analysis disclosed that the smaller alkali cations influence formation and productivity of enzyme-substrate complexes more efficiently than the larger ones. A subsequent systematic investigation of twoLinB mutants with engineered charge in the cation-binding site revealed that the observed cation affinities are enhanced by increasing the number of negatively charged residues at the tunnel mouth, and vice versa, reduced by decreasing t

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Physical Chemistry B

ISSN

1520-6106

e-ISSN

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Volume of the periodical

117

Issue of the periodical within the volume

21

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

6394-6402

UT code for WoS article

000319896800006

EID of the result in the Scopus database

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