Characterization of Neutral Lipase BT-1 Isolated from the Labial Gland of Bombus terrestris Males
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00421832" target="_blank" >RIV/61388963:_____/13:00421832 - isvavai.cz</a>
Alternative codes found
RIV/26296080:_____/13:#0000591
Result on the web
<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0080066" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0080066</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0080066" target="_blank" >10.1371/journal.pone.0080066</a>
Alternative languages
Result language
angličtina
Original language name
Characterization of Neutral Lipase BT-1 Isolated from the Labial Gland of Bombus terrestris Males
Original language description
This is the first report on neutral lipasefrom the cephalic part of B. terrestris labial glands. Purified lipase BT-1 displays broad specificity but preferentially hydrolyzes shorter fatty acid chains (C8-C12) from diacylglycerols and triacylglycerols. The lipase is most active at neutral pH, with a pH optimum of 8.3. The highest level of lipase BT-1 gene and protein expression is in young individuals. In bumblebees older than 3 days, the lipase gene expression level and enzymatic activity are decreased. Interestingly, according to transcript-level RNA seq analysis, the expression of lipase BT-1 is significantly higher in the bumblebee labial gland than in the fat body. The lipase BT-1 gene is the second most abundantly expressed gene in the labial gland of B. terrestris, which suggests important role of this enzyme in this tissue where pheromones are accumulated.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
"e80066/1"-"e80066/11"
UT code for WoS article
000327216200100
EID of the result in the Scopus database
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