Using cryoEM Reconstruction and Phase Extension to Determine Crystal Structure of Bacteriophage .fi.6 Major Capsid Protein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00421902" target="_blank" >RIV/61388963:_____/13:00421902 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10930-013-9526-x" target="_blank" >http://dx.doi.org/10.1007/s10930-013-9526-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10930-013-9526-x" target="_blank" >10.1007/s10930-013-9526-x</a>
Alternative languages
Result language
angličtina
Original language name
Using cryoEM Reconstruction and Phase Extension to Determine Crystal Structure of Bacteriophage .fi.6 Major Capsid Protein
Original language description
Bacteriophage I center dot 6 is a double-stranded RNA virus that has been extensively studied as a model organism. Here we describe structure determination of I center dot 6 major capsid protein P1. The protein crystallized in base centered orthorhombicspace group C222(1). Matthews's coefficient indicated that the crystals contain from four to seven P1 subunits in the crystallographic asymmetric unit. The self-rotation function had shown presence of fivefold axes of non-crystallographic symmetry in thecrystals. Thus, electron density map corresponding to a P1 pentamer was excised from a previously determined cryoEM reconstruction of the I center dot 6 procapsid at 7 resolution and used as a model for molecular replacement. The phases for reflectionsat higher than 7 resolution were obtained by phase extension employing the fivefold non-crystallographic symmetry present in the crystal. The averaged 3.6 -resolution electron density map was of sufficient quality to allow model building.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protein Journal
ISSN
1572-3887
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
635-640
UT code for WoS article
000328080300006
EID of the result in the Scopus database
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