Precise estimation of HPHT nanodiamond size distribution based on transmission electron microscopy image analysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00429945" target="_blank" >RIV/61388963:_____/14:00429945 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.diamond.2014.04.002" target="_blank" >http://dx.doi.org/10.1016/j.diamond.2014.04.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.diamond.2014.04.002" target="_blank" >10.1016/j.diamond.2014.04.002</a>

Result language

angličtina

Original language name

Precise estimation of HPHT nanodiamond size distribution based on transmission electron microscopy image analysis

Original language description

Fluorescent nanodiamonds (NDs) are extremely promising fluorescent probes and sensors in experimental biology. For these applications, NDs obtained from high-pressure, high-temperature (HPHT) synthesis are used. Commercial HPHT NDs are polydisperse and of irregular shape. In this work, we used image analysis of transmission electron microscopy (TEM) micrographs to determine the actual size distribution of an HPHT ND sample with high precision. We developed novel TEM sample preparation procedure, which includes hydrophilization of a carbon-coated TEM grid by UV/ozone treatment and subsequent charge reversal and charge-controlled adsorption of nanoparticles. This method results in NDs spread evenly on the grid and radically improves the quality of TEM samples. Image analysis of TEM micrographs of commercial NDs revealed an unexpectedly high abundance of sub-10 nm NDs in the sample. This method can also be used for preparing TEM samples of other negatively charged nanoparticles, such as c

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CC - Organic chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Diamond and Related Materials

ISSN

0925-9635

e-ISSN

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Volume of the periodical

46

Issue of the periodical within the volume

Jun

Country of publishing house

CH - SWITZERLAND

Number of pages

4

Pages from-to

21-24

UT code for WoS article

000337985900004

EID of the result in the Scopus database

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