Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid- Ordered and Liquid-Disordered Phases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00431198" target="_blank" >RIV/61388963:_____/14:00431198 - isvavai.cz</a>

Alternative codes found

RIV/61388955:_____/14:00431198

Result on the web

<a href="http://dx.doi.org/10.1016/j.bpj.2014.07.018" target="_blank" >http://dx.doi.org/10.1016/j.bpj.2014.07.018</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bpj.2014.07.018" target="_blank" >10.1016/j.bpj.2014.07.018</a>

Result language

angličtina

Original language name

Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid- Ordered and Liquid-Disordered Phases

Original language description

Cholesterol is important for the formation of microdomains in supported lipid bilayers and is enriched in the liquidordered phase. To understand the interactions leading to this enrichment, we developed an AFM-based single-lipid-extraction (SLX) approachthat enables us to determine the anchoring strength of cholesterol in the two phases of a phase-separated lipid membrane. As expected, the forces necessary for extracting a single cholesterol molecule from liquid-ordered phases are significantly higherthan for extracting it from the liquid-disordered phases. Interestingly, application of the Bell model shows two energy barriers that correlate with the head and full length of the cholesterol molecule. The resulting lifetimes for complete extraction are90 s and 11 s in the liquid-ordered and liquid-disordered phases, respectively. Molecular dynamics simulations of the very same experiment show similar force profiles and indicate that the stabilization of cholesterol in the liquid-order

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

<a href="/en/project/GBP208%2F12%2FG016" target="_blank" >GBP208/12/G016: Controlling structure and function of biomolecules at the molecular scale: theory meets experiment</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biophysical Journal

ISSN

0006-3495

e-ISSN

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Volume of the periodical

107

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

1167-1175

UT code for WoS article

000341275100017

EID of the result in the Scopus database

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