Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid- Ordered and Liquid-Disordered Phases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00431198" target="_blank" >RIV/61388963:_____/14:00431198 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/14:00431198
Result on the web
<a href="http://dx.doi.org/10.1016/j.bpj.2014.07.018" target="_blank" >http://dx.doi.org/10.1016/j.bpj.2014.07.018</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpj.2014.07.018" target="_blank" >10.1016/j.bpj.2014.07.018</a>
Alternative languages
Result language
angličtina
Original language name
Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid- Ordered and Liquid-Disordered Phases
Original language description
Cholesterol is important for the formation of microdomains in supported lipid bilayers and is enriched in the liquidordered phase. To understand the interactions leading to this enrichment, we developed an AFM-based single-lipid-extraction (SLX) approachthat enables us to determine the anchoring strength of cholesterol in the two phases of a phase-separated lipid membrane. As expected, the forces necessary for extracting a single cholesterol molecule from liquid-ordered phases are significantly higherthan for extracting it from the liquid-disordered phases. Interestingly, application of the Bell model shows two energy barriers that correlate with the head and full length of the cholesterol molecule. The resulting lifetimes for complete extraction are90 s and 11 s in the liquid-ordered and liquid-disordered phases, respectively. Molecular dynamics simulations of the very same experiment show similar force profiles and indicate that the stabilization of cholesterol in the liquid-order
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP208%2F12%2FG016" target="_blank" >GBP208/12/G016: Controlling structure and function of biomolecules at the molecular scale: theory meets experiment</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biophysical Journal
ISSN
0006-3495
e-ISSN
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Volume of the periodical
107
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
1167-1175
UT code for WoS article
000341275100017
EID of the result in the Scopus database
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