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Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid- Ordered and Liquid-Disordered Phases

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00431198" target="_blank" >RIV/61388963:_____/14:00431198 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388955:_____/14:00431198

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.bpj.2014.07.018" target="_blank" >http://dx.doi.org/10.1016/j.bpj.2014.07.018</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.bpj.2014.07.018" target="_blank" >10.1016/j.bpj.2014.07.018</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid- Ordered and Liquid-Disordered Phases

  • Original language description

    Cholesterol is important for the formation of microdomains in supported lipid bilayers and is enriched in the liquidordered phase. To understand the interactions leading to this enrichment, we developed an AFM-based single-lipid-extraction (SLX) approachthat enables us to determine the anchoring strength of cholesterol in the two phases of a phase-separated lipid membrane. As expected, the forces necessary for extracting a single cholesterol molecule from liquid-ordered phases are significantly higherthan for extracting it from the liquid-disordered phases. Interestingly, application of the Bell model shows two energy barriers that correlate with the head and full length of the cholesterol molecule. The resulting lifetimes for complete extraction are90 s and 11 s in the liquid-ordered and liquid-disordered phases, respectively. Molecular dynamics simulations of the very same experiment show similar force profiles and indicate that the stabilization of cholesterol in the liquid-order

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CF - Physical chemistry and theoretical chemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GBP208%2F12%2FG016" target="_blank" >GBP208/12/G016: Controlling structure and function of biomolecules at the molecular scale: theory meets experiment</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biophysical Journal

  • ISSN

    0006-3495

  • e-ISSN

  • Volume of the periodical

    107

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    1167-1175

  • UT code for WoS article

    000341275100017

  • EID of the result in the Scopus database