Graphene oxide immobilized enzymes show high thermal and solvent stability

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00443878" target="_blank" >RIV/61388963:_____/15:00443878 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22310/15:43899655
Result on the web
<a href="http://pubs.rsc.org/en/content/articlepdf/2015/nr/c5nr00438a" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2015/nr/c5nr00438a</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c5nr00438a" target="_blank" >10.1039/c5nr00438a</a>

Result language
angličtina
Original language name
Graphene oxide immobilized enzymes show high thermal and solvent stability
Original language description
The thermal and solvent tolerance of enzymes is highly important for their industrial use. We show here that the enzyme lipase from Rhizopus oryzae exhibits exceptionally high thermal stability and high solvent tolerance and even increased activity in acetone when immobilized onto a graphene oxide (GO) nanosupport prepared by Staudenmaier and Brodie methods. We studied various forms of immobilization of the enzyme: by physical adsorption, covalent attachment, and additional crosslinking. The activity recovery was shown to be dependent on the support type, enzyme loading and immobilization procedure. Covalently immobilized lipase showed significantly better resistance to heat inactivation (the activity recovery was 65% at 70 degrees C) in comparison with the soluble counterpart (the activity recovery was 65% at 40 degrees C). Physically adsorbed lipase achieved over 100% of the initial activity in a series of organic solvents. These findings, showing enhanced thermal stability and solve
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Project
<a href="/en/project/GA15-09001S" target="_blank" >GA15-09001S: Chemical modifications of graphene based materials: Synthesis of graphane and halogengraphene</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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