Orchestrating the Biosynthesis of an Unnatural Pyrrolysine Amino Acid for Its Direct Incorporation into Proteins Inside Living Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00444532" target="_blank" >RIV/61388963:_____/15:00444532 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/chem.201500971" target="_blank" >http://dx.doi.org/10.1002/chem.201500971</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chem.201500971" target="_blank" >10.1002/chem.201500971</a>

Result language

angličtina

Original language name

Orchestrating the Biosynthesis of an Unnatural Pyrrolysine Amino Acid for Its Direct Incorporation into Proteins Inside Living Cells

Original language description

We here report the construction of an E. coli expression system able to manufacture an unnatural amino acid by artificial biosynthesis. This can be orchestrated with incorporation into protein by amber stop codon suppression inside a living cell. In ourcase an alkyne-bearing pyrrolysine amino acid was biosynthesized and incorporated site-specifically allowing orthogonal double protein labeling.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CC - Organic chemistry

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry - A European Journal

ISSN

0947-6539

e-ISSN

—

Volume of the periodical

21

Issue of the periodical within the volume

21

Country of publishing house

DE - GERMANY

Number of pages

4

Pages from-to

7701-7704

UT code for WoS article

000354204300010

EID of the result in the Scopus database

2-s2.0-84928952987