Activity-Based Protein Profiling of Rhomboid Proteases in Liposomes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00446560" target="_blank" >RIV/61388963:_____/15:00446560 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/cbic.201500213" target="_blank" >http://dx.doi.org/10.1002/cbic.201500213</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.201500213" target="_blank" >10.1002/cbic.201500213</a>
Alternative languages
Result language
angličtina
Original language name
Activity-Based Protein Profiling of Rhomboid Proteases in Liposomes
Original language description
Although activity-based protein profiling (ABPP) has been used to study a variety of enzyme classes, its application to intramembrane proteases is still in its infancy. Intramembrane proteolysis is an important biochemical mechanism for activating proteins residing within the membrane in a dormant state. Rhomboid proteases (intramembrane serine proteases) are embedded in the lipid bilayers of membranes and occur in all phylogenetic domains. The study of purified rhomboid proteases has mainly been performed in detergent micelle environments. Here we report on the reconstitution of rhomboids in liposomes. Using ABPP, we have been able to detect active rhomboids in large and giant unilamellar vesicles. We have found that the inhibitor profiles of rhomboids in micelles and liposomes are similar, thus validating previous inhibitor screenings. Moreover, fluorescence microscopy experiments on the liposomes constitute the first steps towards activity-based imaging of rhomboid proteases in memb
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chembiochem
ISSN
1439-4227
e-ISSN
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Volume of the periodical
16
Issue of the periodical within the volume
11
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
1616-1621
UT code for WoS article
000358330300012
EID of the result in the Scopus database
2-s2.0-84937251917