Antibacterial effect of compounds of peptide nature contained in aqueous extract of Brassica napus, Solanum lycopersicum and Tetragonia tetragonioides leaves

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00506427" target="_blank" >RIV/61388963:_____/15:00506427 - isvavai.cz</a>

Result on the web

<a href="https://www.jmbfs.org/issue/april-may-2015-vol-4-no/jmbfs-0684-neubauerova/?issue_id=3869&article_id=10" target="_blank" >https://www.jmbfs.org/issue/april-may-2015-vol-4-no/jmbfs-0684-neubauerova/?issue_id=3869&article_id=10</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15414/jmbfs.2015.4.5.427-433" target="_blank" >10.15414/jmbfs.2015.4.5.427-433</a>

Result language

angličtina

Original language name

Antibacterial effect of compounds of peptide nature contained in aqueous extract of Brassica napus, Solanum lycopersicum and Tetragonia tetragonioides leaves

Original language description

Treatment of infections caused by pathogenic bacteria is still harder. Due to increasing number of microbial species resistant against so far invented antibiotics. This presents great problem for public health. One of the potential solutions seems to be antimicrobial peptides. Those peptides are synthetized in all organisms as a part of innate immunity with rapid mode of antimicrobial action. Lot of them have been isolated from bacteria, plants, insects and mammals as well. Our project was aimed on finding such peptides in plant extracts, respectively in leaves of Brassica napus (canola), Solanum lycopersicum (tomato) and Tetragonia tetragonioides (New Zealand spinach). We used several separation techniques to obtain fractions containing compounds of peptide nature with hydrophobic character. Antimicrobial activity of these fractions was tested against several gram-positive and gram-negative bacteria. Mass spectrometry analysis of antimicrobial active fractions proved presence of low molecular peptides with molecular masses 1.9 - 4.9 kDa and a partial amino acid sequence in hydrophobic part of Tetragonia extract. In hydrophilic fraction of the Solanum extract with proved antibacterial activity two patogenesis-related proteins with antifungal activity NP24 and TPM-1 were detected.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microbiology, Biotechnology and Food Sciences

ISSN

1338-5178

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

5

Country of publishing house

SK - SLOVAKIA

Number of pages

7

Pages from-to

427-433

UT code for WoS article

000458093400011

EID of the result in the Scopus database

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