A Rotational BODIPY Nucleotide: An Environment-Sensitive Fluorescence-Lifetime Probe for DNA Interactions and Applications in Live-Cell Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00458614" target="_blank" >RIV/61388963:_____/16:00458614 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/16:00458614 RIV/00216208:11310/16:10323524
Result on the web
<a href="http://dx.doi.org/10.1002/anie.201507922" target="_blank" >http://dx.doi.org/10.1002/anie.201507922</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/anie.201507922" target="_blank" >10.1002/anie.201507922</a>
Alternative languages
Result language
angličtina
Original language name
A Rotational BODIPY Nucleotide: An Environment-Sensitive Fluorescence-Lifetime Probe for DNA Interactions and Applications in Live-Cell Microscopy
Original language description
Fluorescent probes for detecting the physical properties of cellular structures have become valuable tools in life sciences. The fluorescence lifetime of molecular rotors can be used to report on variations in local molecular packing or viscosity. We used a nucleoside linked to a meso-substituted BODIPY fluorescent molecular rotor (dCbdp) to sense changes in DNA microenvironment both in vitro and in living cells. DNA incorporating dCbdp can respond to interactions with DNA-binding proteins and lipids by changes in the fluorescence lifetimes in the range 0.5-2.2 ns. We can directly visualize changes in the local environment of exogenous DNA during transfection of living cells. Relatively long fluorescence lifetimes and extensive contrast for detecting changes in the microenvironment together with good photostability and versatility for DNA synthesis make this probe suitable for analysis of DNA-associated processes, cellular structures, and also DNA-based nanomaterials.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Angewandte Chemie - International Edition
ISSN
1433-7851
e-ISSN
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Volume of the periodical
55
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
174-178
UT code for WoS article
000368065300013
EID of the result in the Scopus database
2-s2.0-84955191541