Polymerase Synthesis of Base-Modified DNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00461380" target="_blank" >RIV/61388963:_____/16:00461380 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/978-3-319-27111-8_6" target="_blank" >http://dx.doi.org/10.1007/978-3-319-27111-8_6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-319-27111-8_6" target="_blank" >10.1007/978-3-319-27111-8_6</a>

Result language

angličtina

Original language name

Polymerase Synthesis of Base-Modified DNA

Original language description

Enzymatic synthesis of base-modified DNA by polymerase incorporation of modified nucleotides is discussed. Modified 20-deoxyribonucleoside triphosphates (dNTPs) are key substrates for polymerases and can be prepared either by triphosphorylation of modified nucleosides or by direct aqueous cross-coupling reactions of halogenated dNTPs with alkynes, arylboronic acids, or alkenes. The methods of polymerase synthesis include primer extension, PCR, nicking enzyme amplifications, and other methods which enable the synthesis of diverse types of long or short and double-stranded DNA or single-stranded oligonucleotides. The applications include labeling in diagnostics (labeling or coding of DNA bases) and chemical biology (bioconjugations, modulation of protein binding, etc.).

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

CC - Organic chemistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Modified Nucleic Acids

ISBN

978-3-319-27109-5

Number of pages of the result

22

Pages from-to

123-144

Number of pages of the book

276

Publisher name

Springer

Place of publication

Cham

UT code for WoS chapter

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