2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00469642" target="_blank" >RIV/61388963:_____/16:00469642 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10330182
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/anie.201609007/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/anie.201609007/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/anie.201609007" target="_blank" >10.1002/anie.201609007</a>
Alternative languages
Result language
angličtina
Original language name
2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove
Original language description
2-Deoxyadenosine triphosphate (dATP) derivatives bearing diverse substituents (Cl, NH2, CH3, vinyl, ethynyl, and phenyl) at position 2 were prepared and tested as substrates for DNA polymerases. The 2-phenyl-dATP was not a substrate for DNA polymerases, but the dATPs bearing smaller substituents were good substrates in primer-extension experiments, producing DNA substituted in the minor groove. The vinyl-modified DNA was applied in thiol-ene addition and the ethynyl-modified DNA was applied in a CuAAC click reaction to form DNA labelled with fluorescent dyes in the minor groove.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA14-04289S" target="_blank" >GA14-04289S: Modifications and bioorthogonal reactions in the major groove of DNA for regulation of protein binding and gene expression</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Angewandte Chemie - International Edition
ISSN
1433-7851
e-ISSN
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Volume of the periodical
55
Issue of the periodical within the volume
51
Country of publishing house
DE - GERMANY
Number of pages
4
Pages from-to
15856-15859
UT code for WoS article
000390599400026
EID of the result in the Scopus database
2-s2.0-85004154271