Synthesis of the derivatives of 6-amino-uracil labelled with C-14

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00471547" target="_blank" >RIV/61388963:_____/16:00471547 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/jlcr.3405" target="_blank" >http://dx.doi.org/10.1002/jlcr.3405</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jlcr.3405" target="_blank" >10.1002/jlcr.3405</a>

Result language
angličtina
Original language name
Synthesis of the derivatives of 6-amino-uracil labelled with C-14
Original language description
The radioactively labelled 6-amino-5-nitroso-uracil (1) and 5-acetyl-6-amino-1,3-dimethyl-uracil (2) were required for metabolic studies to assess their suitability as drug candidates. A common precursor for both compounds was [cyano-C-14] cyanoacetic acid (6), readily prepared from potassium [C-14]cyanide. ACS reagents, namely, diethyl ether, acetic acid and acetic anhydride, had to be rigorously repurified to achieve a successful synthesis of C-14-labelled compounds on a tenth-of-a-milligramme scale. 6-Amino-5-nitroso-[6-C-14] uracil (1-C-14) (0.55 mCi) was prepared with radiochemical purity>98% and specific activity (SA) = 55.6 mCi/mmol. 5-Acetyl-6-amino-1,3-dimethyl-[6-C-14] uracil (2-C-14) (8 mCi) was prepared with radiochemical purity>97% and SA = 55.6 mCi/mmol. It has been shown that a SA assay can be made from standard C-13 NMR spectra, thus avoiding the need to perform lengthier inverse-gated C-13 NMR experiments.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Project
<a href="/en/project/LG13002" target="_blank" >LG13002: Membership in Scientific Comitee of Central European Division of International Isotope Society</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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