Non-uniform self-assembly: On the anisotropic architecture of alpha-synuclein supra-fibrillar aggregates

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00478232" target="_blank" >RIV/61388963:_____/17:00478232 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41598-017-06532-1" target="_blank" >https://www.nature.com/articles/s41598-017-06532-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-017-06532-1" target="_blank" >10.1038/s41598-017-06532-1</a>

Result language

angličtina

Original language name

Non-uniform self-assembly: On the anisotropic architecture of alpha-synuclein supra-fibrillar aggregates

Original language description

Although the function of biopolymer hydrogels in nature depends on structural anisotropy at mesoscopic length scales, the self-assembly of such anisotropic structures in vitro is challenging. Here we show that fibrils of the protein alpha-synuclein spontaneously self-assemble into structurally anisotropic hydrogel particles. While the fibrils in the interior of these supra-fibrillar aggregates (SFAs) are randomly oriented, the fibrils in the periphery prefer to cross neighboring fibrils at high angles. This difference in organization coincides with a significant difference in polarity of the environment in the central and peripheral parts of the SFA. We rationalize the structural anisotropy of SFAs in the light of the observation that alpha S fibrils bind a substantial amount of counterions. We propose that, with the progress of protein polymerization into fibrils, this binding of counterions changes the ionic environment which triggers a change in fibril organization resulting in anisotropy in the architecture of hydrogel particles.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10610 - Biophysics

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

Aug 9

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000407294200056

EID of the result in the Scopus database

2-s2.0-85027136128