Modification of C Terminus Provides New Insights into the Mechanism of alpha-Synuclein Aggregation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00485440" target="_blank" >RIV/61388963:_____/17:00485440 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.bpj.2017.08.027" target="_blank" >http://dx.doi.org/10.1016/j.bpj.2017.08.027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bpj.2017.08.027" target="_blank" >10.1016/j.bpj.2017.08.027</a>

Result language

angličtina

Original language name

Modification of C Terminus Provides New Insights into the Mechanism of alpha-Synuclein Aggregation

Original language description

Aggregation of neuronal protein alpha-synuclein leads to the formation of amyloid fibrils, which are associated with the development of Parkinson's disease. The mechanism of alpha-synuclein pathology is not fully understood and is a subject of active research in the field. To tackle this problem, the fusions of fluorescent proteins to alpha-synuclein C-terminus are often used in cellular and animal studies. The effects induced by such alpha-synuclein sequence extension on alpha-synuclein aggregation propensity are, however, not systematically examined despite the evidence that the negatively charged C-terminus plays a critical role in the regulation of alpha-synuclein aggregation. In this work, we investigated how the charge and length variations of the C-terminus affect the aggregation propensity of alpha-synuclein. To address these questions, we prepared mutants of alpha-synuclein carrying additional moieties of different charge and length at the protein C-terminus. We determined the rates of two different aggregation stages (primary nucleation and elongation) based on a thioflavin T kinetic assay. We observed that all mutants bearing neutrally charged moieties of different length fibrilized slower than wild-type alpha-synuclein. The primary nucleation and elongation rates strongly decreased with increase of the C-terminal extension length. Meanwhile, charge variation of the C-terminus significantly changed the rate of alpha-synuclein nucleation, but did not markedly affect the rate of fibril elongation. Our data demonstrate that both the charge and length of the C-terminus play an important role at the stage of initial fibril formation, but the stage of fibril elongation is affected mainly by the length of C-terminal extension. In addition, our results suggest that there are at least two steps of incorporation of alpha-synuclein monomers into the amyloid fibril: namely, the initial monomer binding to the fibril end (charge-dependent, relatively fast), and the subsequent conformational change of the protein (charge-independent, relatively slow, and thus the rate-limiting step).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10610 - Biophysics

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biophysical Journal

ISSN

0006-3495

e-ISSN

—

Volume of the periodical

113

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

2182-2191

UT code for WoS article

000416213100010

EID of the result in the Scopus database

2-s2.0-85029605070