PI(4,5)P-2 controls plasma membrane PI4P and PS levels via ORP5/8 recruitment to ER-PM contact sites

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00490854" target="_blank" >RIV/61388963:_____/18:00490854 - isvavai.cz</a>
Result on the web
<a href="http://jcb.rupress.org/content/217/5/1797" target="_blank" >http://jcb.rupress.org/content/217/5/1797</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1083/jcb.201710095" target="_blank" >10.1083/jcb.201710095</a>

Result language
angličtina
Original language name
PI(4,5)P-2 controls plasma membrane PI4P and PS levels via ORP5/8 recruitment to ER-PM contact sites
Original language description
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) is a critically important regulatory lipid of the plasma membrane (PM), however, little is known about how cells regulate PM PI(4,5)P-2 levels. Here, we show that the phosphatidylinositol 4-phosphate (PI4P)/phosphatidylserine (PS) transfer activity of the endoplasmic reticulum (ER)-resident ORP5 and ORP8 is regulated by both PM PI4P and PI(4,5)P-2. Dynamic control of ORP5/8 recruitment to the PM occurs through interactions with the N-terminal Pleckstrin homology domains and adjacent basic residues of ORP5/8 with both PI4P and PI(4,5)P-2. Although ORP5 activity requires normal levels of these inositides, ORP8 is called on only when PI(4,5) P-2 levels are increased. Regulation of the ORP5/8 attachment to the PM by both phosphoinositides provides a powerful means to determine the relative flux of PI4P toward the ER for PS transport and Sac1-mediated dephosphorylation and PIP 5-kinase-mediated conversion to PI(4,5)P-2. Using this rheostat, cells can maintain PI(4,5)P-2 levels by adjusting the availability of PI4P in the PM.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GA17-05200S" target="_blank" >GA17-05200S: In-solution study of the lipid kinase PI4KB heteromeric protein complexes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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