Application of capillary electromigration methods for physicochemical measurements
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00492951" target="_blank" >RIV/61388963:_____/18:00492951 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/B978-0-12-809375-7.00024-1" target="_blank" >http://dx.doi.org/10.1016/B978-0-12-809375-7.00024-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/B978-0-12-809375-7.00024-1" target="_blank" >10.1016/B978-0-12-809375-7.00024-1</a>
Alternative languages
Result language
angličtina
Original language name
Application of capillary electromigration methods for physicochemical measurements
Original language description
In this chapter, high-performance capillary electromigration (HPCE or CE) methods are presented as powerful tools for the determination of important physicochemical parameters of (bio)molecules. Using the different HPCE methods (zone electrophoresis in free solutions or in sieving/gel media, isotachophoresis, isoelectric focusing, affinity electrophoresis, and electrokinetic chromatography), the following physicochemical characteristics of a wide spectrum of compounds can be determined: effective, ionic and limiting mobilities, effective charges, isoelectric points, Stokes radii, relative molecular masses, partition constants, diffusion coefficients, and acidity (ionization) constants (pKa). In addition, the binding (stability, association, formation, or dissociation) constants of (bio)molecular complexes and rate constants of chemical and enzymatic reactions and noncovalent molecular interactions can be estimated. Advantages of HPCE methods for these physicochemical measurements include high separation efficiency, short analysis time, and especially the ultra-small mass (picogram range) and volume (nanoliter level) of injected solute samples and low consumption of chemicals and solvents. Moreover, the characterization of not quite pure solutes and solutes sparingly soluble in water is possible.
Czech name
—
Czech description
—
Classification
Type
C - Chapter in a specialist book
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Capillary Electromigration Separation Methods
ISBN
978-0-12-809375-7
Number of pages of the result
45
Pages from-to
547-591
Number of pages of the book
626
Publisher name
Elsevier
Place of publication
Amsterdam
UT code for WoS chapter
000462135300022