Endogenous Fatty Acids Are Essential Signaling Factors of Pancreatic beta-Cells and Insulin Secretion
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00495209" target="_blank" >RIV/61388963:_____/18:00495209 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.2337/db17-1215" target="_blank" >http://dx.doi.org/10.2337/db17-1215</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2337/db17-1215" target="_blank" >10.2337/db17-1215</a>
Alternative languages
Result language
angličtina
Original language name
Endogenous Fatty Acids Are Essential Signaling Factors of Pancreatic beta-Cells and Insulin Secretion
Original language description
The secretion of insulin from beta-cells depends on extracellular factors, in particular glucose and other small molecules, some of which act on G-protein-coupled receptors. Fatty acids (FAs) have been discussed as exogenous secretagogues of insulin for decades, especially after the FA receptor GPR40 (G-protein-coupled receptor 40) was discovered. However, the role of FAs as endogenous signaling factors has not been investigated until now. In the present work, we demonstrate that lowering endogenous FA levels in beta-cell medium by stringent washing or by the application of FA-free (FAF) BSA immediately reduced glucose-induced oscillations of cytosolic Ca2+ ([Ca2+](i) oscillations) in MIN6 cells and mouse primary beta-cells, as well as insulin secretion. Mass spectrometry confirmed BSA-mediated removal of FAs, with palmitic, stearic, oleic, and elaidic acid being the most abundant species. [Ca2+](i) oscillations in MIN6 cells recovered when BSA was replaced by buffer or as FA levels in the supernatant were restored. This was achieved by recombinant lipase-mediated FA liberation from membrane lipids, by the addition of FA-preloaded FAF-BSA, or by the photolysis of cell-impermeant caged FAs. Our combined data support the hypothesis of FAs as essential endogenous signaling factors for beta-cell activity and insulin secretion.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Diabetes
ISSN
0012-1797
e-ISSN
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Volume of the periodical
67
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
1986-1998
UT code for WoS article
000445059100008
EID of the result in the Scopus database
2-s2.0-85054776125