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Synthesis of a Fluorescent Probe for Sensing Multiple Protein States

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00495680" target="_blank" >RIV/61388963:_____/18:00495680 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1002/ejoc.201800524" target="_blank" >http://dx.doi.org/10.1002/ejoc.201800524</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/ejoc.201800524" target="_blank" >10.1002/ejoc.201800524</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Synthesis of a Fluorescent Probe for Sensing Multiple Protein States

  • Original language description

    We synthesized solvatochromic thiol-reactive fluorescent probes 6FM-M and 7AFM-M possessing two-band emission. 7AFM-M was designed to have a smaller dipole moment than 6FM-M and as a result it showed better separation of the two emission bands. Both probes were used to label a cysteine mutant of α-synuclein (αSyn), a presynaptic neuronal protein associated with Parkinson's disease. We investigated the ability of the probes to sense αSyn binding to lipid membranes and αSyn fibrillization, and compared their sensitivity with that of one-band solvatochromic probes. We found that while all the tested probes can discriminate two αSyn states, only the 7AFM-M probe is able to clearly discriminate all three protein states of αSyn: unstructured, membrane-bound and fibrillar αSyn. Finally, using αSyn labelled with 7AFM-M, we demonstrated that high density of αSyn on lipid membranes may lead to the partial membrane destruction with a formation of lipoprotein particles.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10401 - Organic chemistry

Result continuities

  • Project

    <a href="/en/project/GJ18-06255Y" target="_blank" >GJ18-06255Y: New strategy for inhibition of amyloid fibril formation</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    European Journal of Organic Chemistry

  • ISSN

    1434-193X

  • e-ISSN

  • Volume of the periodical

    2018

  • Issue of the periodical within the volume

    37

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    8

  • Pages from-to

    5155-5162

  • UT code for WoS article

    000446662900010

  • EID of the result in the Scopus database

    2-s2.0-85052462248