Synthesis of a Fluorescent Probe for Sensing Multiple Protein States
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00495680" target="_blank" >RIV/61388963:_____/18:00495680 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/ejoc.201800524" target="_blank" >http://dx.doi.org/10.1002/ejoc.201800524</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/ejoc.201800524" target="_blank" >10.1002/ejoc.201800524</a>
Alternative languages
Result language
angličtina
Original language name
Synthesis of a Fluorescent Probe for Sensing Multiple Protein States
Original language description
We synthesized solvatochromic thiol-reactive fluorescent probes 6FM-M and 7AFM-M possessing two-band emission. 7AFM-M was designed to have a smaller dipole moment than 6FM-M and as a result it showed better separation of the two emission bands. Both probes were used to label a cysteine mutant of α-synuclein (αSyn), a presynaptic neuronal protein associated with Parkinson's disease. We investigated the ability of the probes to sense αSyn binding to lipid membranes and αSyn fibrillization, and compared their sensitivity with that of one-band solvatochromic probes. We found that while all the tested probes can discriminate two αSyn states, only the 7AFM-M probe is able to clearly discriminate all three protein states of αSyn: unstructured, membrane-bound and fibrillar αSyn. Finally, using αSyn labelled with 7AFM-M, we demonstrated that high density of αSyn on lipid membranes may lead to the partial membrane destruction with a formation of lipoprotein particles.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
<a href="/en/project/GJ18-06255Y" target="_blank" >GJ18-06255Y: New strategy for inhibition of amyloid fibril formation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Organic Chemistry
ISSN
1434-193X
e-ISSN
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Volume of the periodical
2018
Issue of the periodical within the volume
37
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
5155-5162
UT code for WoS article
000446662900010
EID of the result in the Scopus database
2-s2.0-85052462248