The matrix-assisted laser desorption/ionisation in-source decay of peptides using ion mobility enabled quadrupole-time-of-flight mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00498562" target="_blank" >RIV/61388963:_____/18:00498562 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/rcm.8284" target="_blank" >http://dx.doi.org/10.1002/rcm.8284</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/rcm.8284" target="_blank" >10.1002/rcm.8284</a>
Alternative languages
Result language
angličtina
Original language name
The matrix-assisted laser desorption/ionisation in-source decay of peptides using ion mobility enabled quadrupole-time-of-flight mass spectrometry
Original language description
Rationale In-source decay (ISD) matrix-assisted laser desorption/ionisation (MALDI) mass spectrometry with a 1,5-diaminonaphthalene (1,5-DAN) matrix is used for the structural characterisation of peptides. However, MALDI spectra are intrinsically complicated by the presence of matrix ions, which interfere with the peptide fragments. This may cause false-positive results or reduced sequence coverage. This paper reports investigations of ISD processes in an intermediate pressure MALDI ion source and a protocol for the removal of interfering ions using ion mobility separation (IMS). Methods An intermediate pressure MALDI source of a Q-IMS-Q-TOF instrument (Synapt G2) has been employed for the ISD of selected peptides using a 1,5-DAN matrix. Results Successful coupling of the MALDI source tuned for ISD experiments using IMS is demonstrated. The IMS made it possible to remove interfering matrix ions effectively from the spectra and thus to increase the confidence of spectral interpretation. Extensive fragment series corresponding to N-C-alpha bond cleavages were observed under optimised conditions, on the other hand, weaker series of ions caused by peptide bond cleavages were prevalent for default conditions and/or the alpha-hydroxycinnamic acid matrix. Conclusions Ion mobility has been used for the elimination of matrix ions. The technique has been applied to top-down sequencing of non-tryptic peptides, such as the human palmitoylated analogue of prolactin-releasing peptide used in recent obesity studies, and human and insect antimicrobial peptides.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA16-01639S" target="_blank" >GA16-01639S: Micro- and nanoflow atmospheric pressure ionizations for bioanalytical mass spectrometry</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Rapid Communications in Mass Spectrometry
ISSN
0951-4198
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
24
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
2099-2105
UT code for WoS article
000450358300001
EID of the result in the Scopus database
2-s2.0-85056594912