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Dual redox labeling of DNA as a tool for electrochemical detection of p53 protein-DNA interactions

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00501012" target="_blank" >RIV/61388963:_____/19:00501012 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081707:_____/19:00501012 RIV/00216224:14740/19:00108148 RIV/00216208:11310/19:10394770

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.aca.2018.10.053" target="_blank" >http://dx.doi.org/10.1016/j.aca.2018.10.053</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.aca.2018.10.053" target="_blank" >10.1016/j.aca.2018.10.053</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Dual redox labeling of DNA as a tool for electrochemical detection of p53 protein-DNA interactions

  • Original language description

    We present a novel dual redox labeling approach enabling a facile relative evaluation of protein-DNA interactions based on immunoprecipitation at magnetic beads (MBIP) with subsequent electro-chemical detection. DNA probes labeled with two different electroactive markers, benzofurazane and nitrobenzene, which yield reduction peaks at distinct potentials, were synthesized using primer extension (PEX) reaction. We show that using the labeled DNA probes, specific and non-specific binding of the p53 protein can be distinguished in a simple competition binding experiment, as a strong preference of the p53 protein was observed towards DNA probes bearing a specific p53 binding site (p53CON), which is in agreement with known binding properties of the p53 protein. The p53 binding to the individual DNA probes can be modulated by specific monoclonal antibodies used for the immuno-precipitation. This approach can potentially be applied, after selection of appropriate DNA probes and monoclonal antibodies, for investigations of DNA-binding properties of other proteins and thus represents a versatile tool for studies of any DNA-binding proteins. (c) 2018 Elsevier B.V. All rights reserved.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytica Chimica Acta

  • ISSN

    0003-2670

  • e-ISSN

  • Volume of the periodical

    1050

  • Issue of the periodical within the volume

    Mar 7

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    9

  • Pages from-to

    123-131

  • UT code for WoS article

    000455933400014

  • EID of the result in the Scopus database

    2-s2.0-85055755902