Phosphatidylinositol 4-kinase III beta (PI4KB) forms highly flexible heterocomplexes that include ACBD3, 14-3-3, and Rab11 proteins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00501576" target="_blank" >RIV/61388963:_____/19:00501576 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-018-37158-6" target="_blank" >https://www.nature.com/articles/s41598-018-37158-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-018-37158-6" target="_blank" >10.1038/s41598-018-37158-6</a>
Alternative languages
Result language
angličtina
Original language name
Phosphatidylinositol 4-kinase III beta (PI4KB) forms highly flexible heterocomplexes that include ACBD3, 14-3-3, and Rab11 proteins
Original language description
Phosphatidylinositol 4-kinase III beta (PI4KB) is a key enzyme of the Golgi system because it produces its lipid hallmark the phosphatidylinositol 4-phosphate (PI4P). It is recruited to Golgi by the Golgi resident ACBD3 protein, regulated by 14-3-3 proteins and it also serves as an adaptor because it recruits the small GTPase Rab11. Here, we analyzed the protein complexes formed by PI4KB in vitro using small angle x-ray scattering (SAXS) and we discovered that these protein complexes are highly flexible. The 14-3-3:PI4KB:Rab11 protein complex has 2:1:1 stoichiometry and its different conformations are rather compact, however, the ACBD3:PI4KB protein complex has both, very compact and very extended conformations. Furthermore, in vitro reconstitution revealed that the membrane is necessary for the formation of ACBD3:PI4KB:Rab11 protein complex at physiological (nanomolar) concentrations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA17-05200S" target="_blank" >GA17-05200S: In-solution study of the lipid kinase PI4KB heteromeric protein complexes</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
Jan 24
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
567
UT code for WoS article
000456554600006
EID of the result in the Scopus database
2-s2.0-85060556364