Effects of sulfation and the environment on the structure of chondroitin sulfate studied via Raman optical activity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00504471" target="_blank" >RIV/61388963:_____/19:00504471 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11320/19:10406255

Result on the web

<a href="https://pubs.rsc.org/en/content/articlelanding/2019/CP/C9CP00472F#!divAbstract" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2019/CP/C9CP00472F#!divAbstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c9cp00472f" target="_blank" >10.1039/c9cp00472f</a>

Result language

angličtina

Original language name

Effects of sulfation and the environment on the structure of chondroitin sulfate studied via Raman optical activity

Original language description

Glycosaminoglycans are linear carbohydrate polymers with essential roles in many biological processes. Chondroitin sulfate (CS) is one of them, omnipresent in living organisms as an important structural component of cartilage. It provides much of its resistance to compression. Despite its biological importance, little is still known about the relation of the CS structure to chemical composition and interaction with the environment. We therefore measured Raman and Raman optical activity (ROA) spectra of five CS samples of different biological origin and variously sulfated CS building blocks (GlcA, GalNAc, and basic disaccharide units) in a wide frequency range between 200 cm(-1) and 1800 cm(-1) and analyzed them with respect to specific structure marker bands. We show that ROA spectroscopy is sensitive to the conformational stability and rigidity of pyranose rings of saccharides, the orientation of sugar hydroxyl groups and the secondary structure of the CS's backbone. The CS secondary structure has been found to be quite stable, with a minor variation as a reaction to physicochemical parameters (concentration, pH, temperature, and the presence of cations). Larger changes were observed under chemical changes (sulfation) of the CS chain. ROA spectroscopy thus exhibited useful potential to study the structure of similar biopolymers.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10403 - Physical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physical Chemistry Chemical Physics

ISSN

1463-9076

e-ISSN

—

Volume of the periodical

21

Issue of the periodical within the volume

14

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

7367-7377

UT code for WoS article

000464580600011

EID of the result in the Scopus database

2-s2.0-85063994384