Electrochemical genosensor for the direct detection of tailed PCR amplicons incorporating ferrocene labelled dATP

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00504622" target="_blank" >RIV/61388963:_____/19:00504622 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10394787
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0956566319302751?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0956566319302751?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bios.2019.03.060" target="_blank" >10.1016/j.bios.2019.03.060</a>

Result language
angličtina
Original language name
Electrochemical genosensor for the direct detection of tailed PCR amplicons incorporating ferrocene labelled dATP
Original language description
An electrochemical genosensor for the detection and quantification of Karlodinium armiger is presented. The genosensor exploits tailed primers and ferrocene labelled dATP analogue to produce PCR products that can be directly hybridised on a gold electrode array and quantitatively measured using square wave voltammetry. Tailed primers consist of a sequence specific for the target, followed by a carbon spacer and a sequence specifically designed not to bind to genomic DNA, resulting in a duplex flanked by single stranded binding primers. The incorporation of the 7-(ferrocenylethynyl)-7-deaza-2'-deoxyadenosine triphosphate was optimised in terms of a compromise between maximum PCR efficiency and the limit of detection and sensitivity attainable using electrochemical detection via hybridisation of the tailed, ferrocene labelled PCR product. A limit of detection of 277aM with a linear range from 315aM to 10 fM starting DNA concentration and a sensitivity of 122 nA decade(-1) was achieved. The system was successfully applied to the detection of genomic DNA in real seawater samples.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
<a href="/en/project/EF16_019%2F0000729" target="_blank" >EF16_019/0000729: Chemical biology for drugging undruggable targets</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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