Inhibitory activity of Scorzonera latifolia and its components on enzymes connected with healing process

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00509837" target="_blank" >RIV/61388963:_____/19:00509837 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16370/19:43877337

Result on the web

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0378874118324528?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0378874118324528?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jep.2019.112168" target="_blank" >10.1016/j.jep.2019.112168</a>

Result language

angličtina

Original language name

Inhibitory activity of Scorzonera latifolia and its components on enzymes connected with healing process

Original language description

Ethnopharmacological relevance: Scorzonera latifolia (Fisch. & Mey.) DC. (Asteraceae) grows naturally in Eastern Anatolia, northeastern Iran, and Caucasus. Latex of S. latifolia roots is used in Turkish folk medicine for its analgesic effects, externally to cure infertility in women, and internally as an antihelmintic. The milk obtained from the stem of the Scorzonera species is used for wound healing activity. Antinociceptive, anti-inflammatory, wound-healing, antioxidant, and antimicrobial activities have previously been reported for S. latifolia. Aim of the study: A methanol extract of the aerial parts of Scorzonera latifolia that had been shown to possess wound-healing activity, was used to elucidate the possible mechanism of the wound-healing activity and to identify the compound(s) responsible for the effect by means of bioassay-guided fractionation. Materials and methods: The wound-healing activity potential of methanol extract of S. latifolia was detected by evaluating the inhibitory activity on the collagenase, hyaluronidase and elastase, which play important roles in the wound-healing process. Succesive fractionation of the methanol extract using petroleum ether, chloroform, ethyl acetate, respectively, and the residual wateryielded four respective fractions. The ethyl acetate part, which was determined as the most active fraction, was selected for further separation using chromatographic techniques. Results: Ethylacetate fraction exhibited significant inhibitory activities on collagenase and elastase. Chromatographic separation of the ethylacetate extract yielded an active subfraction, from which was used to isolate quercetin-3-O-beta-apiofuranosyl-(1’’’-> 2’’)-beta-D-glucopyranoside (1), quercetin-3-O-alpha-rhamnopyranosyl(1 -> 6)-beta-D-galactopyranoside (2), isoorientin (3), and 7-methylisoorientin (4). Of the compounds tested, 7-methylisoorientin (4) exerted inhibitory activity on collagenase and elastase, while quercetin-3-O-beta-apiofuranosyl-(1’’’ -> 2’’)-beta-glucopyranoside (1) inhibited collagenase only. None of the fractions, or isolated compounds showed any inhibitory effect on hyaluronidase. It must be mentioned, that in vitro tests showed that compounds 1-4 inhibit the collagenase and elastase and could help wound-healing process. However, the inhibititory effect of the methanol extract appears to be greater than that of both of the ethylacetate fraction, subfraction G and the isolated compounds, which suggest that a synergistic interaction of several compounds could be responsible for the wound-healing activity of the aerial parts of S. latifolia.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10401 - Organic chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Ethnopharmacology

ISSN

0378-8741

e-ISSN

—

Volume of the periodical

245

Issue of the periodical within the volume

Dec 5

Country of publishing house

IE - IRELAND

Number of pages

7

Pages from-to

112168

UT code for WoS article

000488331300008

EID of the result in the Scopus database

2-s2.0-85071337585