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Method of determination of the ability of substances to bind to analyte active sites

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00520756" target="_blank" >RIV/61388963:_____/19:00520756 - isvavai.cz</a>

  • Result on the web

    <a href="https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=3177931B1&KC=B1&FT=D&ND=4&date=20190501&DB=&locale=en_EP#" target="_blank" >https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=3177931B1&KC=B1&FT=D&ND=4&date=20190501&DB=&locale=en_EP#</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Method of determination of the ability of substances to bind to analyte active sites

  • Original language description

    The invention provides a method for detection of active form of analytes in a sample and/or for determination of ability of tested substances to bind to the active site of these analytes, comprising the following steps: a) analyte or group of analytes from the sample is immobilized on the surface of a solid carrier either by non-specific non-covalent adsorption or by covalent binding of surface functional groups of the analyte and corresponding functional groups of the solid carrier, or preferably via a binding molecule which is bound to the surface of the solid carrier before immobilization of the analyte or group of analytes and is capable of selectively binding the analyte or group of analytes contained in the sample during incubation of the solid carrier with the sample, b) analyte or group of analytes is incubated with a detection probe which binds selectively to the analyte or group of analytes via a compound for selective binding to the analyte active site, whereas the probe consists of a low molecular compound for selective binding to the analyte active site, an oligonucleotide tag, optionally with a covalently attached fluorophore, biotin or a chemical group, and a chemical linker covalently linking the compound for selective binding to the analyte active site and the oligonucleotide tag, c) then the solid carrier is washed to remove unbound detection probe, and subsequently, the amount of bound detection probe is determined, whereas this amount is directly proportional to the amount of the analyte or group of analytes in the sample. The described method has broad application in medicine. Given the exceptional sensitivity of only a few dozen molecules, it provides the ability to determine the protein markers in blood in a concentration yet undetectable.

  • Czech name

  • Czech description

Classification

  • Type

    P - Patent

  • CEP classification

  • OECD FORD branch

    10609 - Biochemical research methods

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Patent/design ID

    EP3177931

  • Publisher

    EPO_1 -

  • Publisher name

    European Patent Office

  • Place of publication

    Munich, The Hague, Berlin, Vienna, Brussels

  • Publication country

  • Date of acceptance

    May 1, 2019

  • Owner name

    Ústav organické chemie a biochemie AV ČR, v. v. i

  • Method of use

    B - Výsledek je využíván orgány státní nebo veřejné správy

  • Usage type

    A - K využití výsledku jiným subjektem je vždy nutné nabytí licence