Method of determination of the ability of substances to bind to analyte active sites

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00520756" target="_blank" >RIV/61388963:_____/19:00520756 - isvavai.cz</a>

Result on the web

<a href="https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=3177931B1&KC=B1&FT=D&ND=4&date=20190501&DB=&locale=en_EP#" target="_blank" >https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=3177931B1&KC=B1&FT=D&ND=4&date=20190501&DB=&locale=en_EP#</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Method of determination of the ability of substances to bind to analyte active sites

Original language description

The invention provides a method for detection of active form of analytes in a sample and/or for determination of ability of tested substances to bind to the active site of these analytes, comprising the following steps: a) analyte or group of analytes from the sample is immobilized on the surface of a solid carrier either by non-specific non-covalent adsorption or by covalent binding of surface functional groups of the analyte and corresponding functional groups of the solid carrier, or preferably via a binding molecule which is bound to the surface of the solid carrier before immobilization of the analyte or group of analytes and is capable of selectively binding the analyte or group of analytes contained in the sample during incubation of the solid carrier with the sample, b) analyte or group of analytes is incubated with a detection probe which binds selectively to the analyte or group of analytes via a compound for selective binding to the analyte active site, whereas the probe consists of a low molecular compound for selective binding to the analyte active site, an oligonucleotide tag, optionally with a covalently attached fluorophore, biotin or a chemical group, and a chemical linker covalently linking the compound for selective binding to the analyte active site and the oligonucleotide tag, c) then the solid carrier is washed to remove unbound detection probe, and subsequently, the amount of bound detection probe is determined, whereas this amount is directly proportional to the amount of the analyte or group of analytes in the sample. The described method has broad application in medicine. Given the exceptional sensitivity of only a few dozen molecules, it provides the ability to determine the protein markers in blood in a concentration yet undetectable.

Czech name

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Czech description

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Type

P - Patent

CEP classification

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OECD FORD branch

10609 - Biochemical research methods

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Patent/design ID

EP3177931

Publisher

EPO_1 -

Publisher name

European Patent Office

Place of publication

Munich, The Hague, Berlin, Vienna, Brussels

Publication country

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Date of acceptance

May 1, 2019

Owner name

Ústav organické chemie a biochemie AV ČR, v. v. i

Method of use

B - Výsledek je využíván orgány státní nebo veřejné správy

Usage type

A - K využití výsledku jiným subjektem je vždy nutné nabytí licence