The bio-chemically selective interaction of hydrogenated and oxidized ultra-small nanodiamonds with proteins and cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00523791" target="_blank" >RIV/61388963:_____/20:00523791 - isvavai.cz</a>
Alternative codes found
RIV/68378271:_____/20:00523791 RIV/68407700:21230/20:00342539 RIV/00216208:11110/20:10410730 RIV/00216208:11140/20:10410730 and 2 more
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0008622320302074" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0008622320302074</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.carbon.2020.02.061" target="_blank" >10.1016/j.carbon.2020.02.061</a>

Result language
angličtina
Original language name
The bio-chemically selective interaction of hydrogenated and oxidized ultra-small nanodiamonds with proteins and cells
Original language description
Ultra-small nanoparticles of a size smaller than or comparable to cell membrane pores (1–5 nm) offer significant potential in the field of biomedicine. This study presents a systematic in vitro investigation of fundamental bio-chemical interactions of such ultra-small hydrogenated and oxidized detonation nanodiamonds (DNDs) with biomolecules and human cells. We apply mass spectrometry methods (LC-MS/MS) for the qualitative and quantitative analysis of the protein corona as a function of the surface chemistry and size of DNDs. We observe that protein interactions with DNDs are more related to their surface chemistry (H/O-termination) rather than size. Bioinformatics characterization of the identified proteins points to the strong influence of electrostatic interaction between proteins and DNDs depending on their termination. Such specific interaction leads to formation of different protein corona on 2 nm DNDs, which influences also interaction with cells including different level of cytotoxicity.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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