Defining the subcellular distribution and metabolic channeling of phosphatidylinositol

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00523809" target="_blank" >RIV/61388963:_____/20:00523809 - isvavai.cz</a>
Result on the web
<a href="https://rupress.org/jcb/article/doi/10.1083/jcb.201906130/133809/Defining-the-subcellular-distribution-and" target="_blank" >https://rupress.org/jcb/article/doi/10.1083/jcb.201906130/133809/Defining-the-subcellular-distribution-and</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1083/jcb.201906130" target="_blank" >10.1083/jcb.201906130</a>

Result language
angličtina
Original language name
Defining the subcellular distribution and metabolic channeling of phosphatidylinositol
Original language description
Phosphatidylinositol (PI) is an essential structural component of eukaryotic membranes that also serves as the common precursor for polyphosphoinositide (PPIn) lipids. Despite the recognized importance of PPIn species for signal transduction and membrane homeostasis, there is still a limited understanding of the relationship between PI availability and the turnover of subcellular PPIn pools. To address these shortcomings, we established a molecular toolbox for investigations of PI distribution within intact cells by exploiting the properties of a bacterial enzyme, PI-specific PLC (PI-PLC). Using these tools, we find a minor presence of PI in membranes of the ER, as well as a general enrichment within the cytosolic leaflets of the Golgi complex, peroxisomes, and outer mitochondrial membrane, but only detect very low steady-state levels of PI within the plasma membrane (PM) and endosomes. Kinetic studies also demonstrate the requirement for sustained PI supply from the ER for the maintenance of monophosphorylated PPIn species within the PM, Golgi complex, and endosomal compartments.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/EF16_019%2F0000729" target="_blank" >EF16_019/0000729: Chemical biology for drugging undruggable targets</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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