A radioligand binding assay for the insulin-like growth factor 2 receptor

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00531943" target="_blank" >RIV/61388963:_____/20:00531943 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10421322
Result on the web
<a href="https://doi.org/10.1371/journal.pone.0238393" target="_blank" >https://doi.org/10.1371/journal.pone.0238393</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0238393" target="_blank" >10.1371/journal.pone.0238393</a>

Result language
angličtina
Original language name
A radioligand binding assay for the insulin-like growth factor 2 receptor
Original language description
Here we produced His-tagged domain 11 (D11), an IGF2-binding element of IGF2R, we immobilized it on the solid support through a well-defined sandwich, consisting of neutravidin, biotin and synthetic anti-His-tag antibodies. Next, we prepared specifically radiolabeled [125I]-monoiodotyrosyl-Tyr2-IGF2 and optimized a sensitive and robust competitive radioligand binding assay for determination of the nanomolar binding affinities of hormones for D11 of IGF2. The assay will be helpful for the characterization of new IGF2 mutants to study the functions of IGF2R and the development of new compounds for the treatment of neurological disorders.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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