Nucleotide‐Bearing Benzylidene‐Tetrahydroxanthylium Near‐IR Fluorophore for Sensing DNA Replication, Secondary Structures and Interactions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00532176" target="_blank" >RIV/61388963:_____/20:00532176 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10420648
Result on the web
<a href="https://doi.org/10.1002/chem.202003192" target="_blank" >https://doi.org/10.1002/chem.202003192</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/chem.202003192" target="_blank" >10.1002/chem.202003192</a>
Alternative languages
Result language
angličtina
Original language name
Nucleotide‐Bearing Benzylidene‐Tetrahydroxanthylium Near‐IR Fluorophore for Sensing DNA Replication, Secondary Structures and Interactions
Original language description
Thymidine triphosphate bearing benzylidene‐tetrahydroxanthylium near‐IR fluorophore linked to the 5‐methyl group via triazole was synthesized through the CuAAC reaction and was used for polymerase synthesis of labelled DNA probes. The fluorophore lights up upon incorporation to DNA (up to 348‐times) presumably due to interactions in major groove and the fluorescence further increases in the single‐stranded oligonucleotide. The labelled dsDNA senses binding of small molecules and proteins by a strong decrease of fluorescence. The nucleotide was used as a light‐up building block in real‐time PCR for detection of SARS‐CoV‐2 virus.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemistry - A European Journal
ISSN
0947-6539
e-ISSN
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Volume of the periodical
26
Issue of the periodical within the volume
52
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
11950-11954
UT code for WoS article
000559973200001
EID of the result in the Scopus database
2-s2.0-85089500940