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Fluorescent Probe for Selective Imaging of α-Synuclein Fibrils in Living Cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00542333" target="_blank" >RIV/61388963:_____/21:00542333 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1021/acschemneuro.1c00090" target="_blank" >https://doi.org/10.1021/acschemneuro.1c00090</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1021/acschemneuro.1c00090" target="_blank" >10.1021/acschemneuro.1c00090</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Fluorescent Probe for Selective Imaging of α-Synuclein Fibrils in Living Cells

  • Original language description

    Plaques of amyloid fibrils composed of neuronal protein α-synuclein are one of the hallmarks of Parkinson’s disease, and their selective imaging is crucial to study the mechanism of its pathogenesis. However, the existing fluorescent probes for amyloids are efficient only in solution and tissue systems, and they are not selective enough for the visualization of amyloid fibrils in living cells. In this study, we present two molecular rotor-based probes RB1 and RB2. These thiazolium probes show affinity to α-synuclein fibrils and turn-on fluorescence response upon interactions. Because of its extended π-conjugation and high rotational degree of freedom, RB1 exhibits a 76 nm red-shift of absorption maxima and 112-fold fluorescence enhancement upon binding to amyloid fibrils. Owing to its strong binding affinity to α-synuclein fibrils, RB1 can selectively stain them in the cytoplasm of living HeLa and SH-SY5Y cells with high optical contrast. RB1 is a cell-permeable and noncytotoxic probe. Taken together, we have demonstrated that RB1 is an amyloid probe with an outstanding absorption red-shift that can be used for intracellular imaging of α-synuclein fibrils.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10610 - Biophysics

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    ACS Chemical Neuroscience

  • ISSN

    1948-7193

  • e-ISSN

    1948-7193

  • Volume of the periodical

    12

  • Issue of the periodical within the volume

    8

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    6

  • Pages from-to

    1293-1298

  • UT code for WoS article

    000643598600002

  • EID of the result in the Scopus database

    2-s2.0-85104917599