High-Throughput Fluorescent Assay for Inhibitor Screening of Proteases from RNA Viruses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00544070" target="_blank" >RIV/61388963:_____/21:00544070 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3390/molecules26133792" target="_blank" >https://doi.org/10.3390/molecules26133792</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules26133792" target="_blank" >10.3390/molecules26133792</a>
Alternative languages
Result language
angličtina
Original language name
High-Throughput Fluorescent Assay for Inhibitor Screening of Proteases from RNA Viruses
Original language description
Spanish flu, polio epidemics, and the ongoing COVID-19 pandemic are the most profound examples of severe widespread diseases caused by RNA viruses. The coronavirus pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands affordable and reliable assays for testing antivirals. To test inhibitors of viral proteases, we have developed an inexpensive high-throughput assay based on fluorescent energy transfer (FRET). We assayed an array of inhibitors for papain-like protease from SARS-CoV-2 and validated it on protease from the tick-borne encephalitis virus to emphasize its versatility. The reaction progress is monitored as loss of FRET signal of the substrate. This robust and reproducible assay can be used for testing the inhibitors in 96- or 384-well plates.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000729" target="_blank" >EF16_019/0000729: Chemical biology for drugging undruggable targets</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecules
ISSN
1420-3049
e-ISSN
1420-3049
Volume of the periodical
26
Issue of the periodical within the volume
13
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
3792
UT code for WoS article
000671975600001
EID of the result in the Scopus database
2-s2.0-85109099042