Anchored linear oligonucleotides: the effective tool for the real-time measurement of uracil DNA glycosylase activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00547495" target="_blank" >RIV/61388963:_____/21:00547495 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/21:73607669 RIV/67985882:_____/21:00547495
Result on the web
<a href="https://doi.org/10.1098/rsob.210136" target="_blank" >https://doi.org/10.1098/rsob.210136</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1098/rsob.210136" target="_blank" >10.1098/rsob.210136</a>
Alternative languages
Result language
angličtina
Original language name
Anchored linear oligonucleotides: the effective tool for the real-time measurement of uracil DNA glycosylase activity
Original language description
Base excision repair is one of the important DNA repair mechanisms in cells. The fundamental role in this complex process is played by DNA glycosylases. Here, we present a novel approach for the real-time measurement of uracil DNA glycosylase activity, which employs selected oligonucleotides immobilized on the surface of magnetic nanoparticles and Förster resonance energy transfer. We also show that the approach can be performed by surface plasmon resonance sensor technology. We demonstrate that the immobilization of oligonucleotides provides much more reliable data than the free oligonucleotides including molecular beacons. Moreover, our results show that the method provides the possibility to address the relationship between the efficiency of uracil DNA glycosylase activity and the arrangement of the used oligonucleotide probes. For instance, the introduction of the nick into oligonucleotide containing the target base (uracil) resulted in the substantial decrease of uracil DNA glycosylase activity of both the bacterial glycosylase and glycosylases naturally present in nuclear lysates.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Open Biology
ISSN
2046-2441
e-ISSN
2046-2441
Volume of the periodical
11
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
210136
UT code for WoS article
000708618300001
EID of the result in the Scopus database
2-s2.0-85119393090