A ubiquitous disordered protein interaction module orchestrates transcription elongation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00548921" target="_blank" >RIV/61388963:_____/21:00548921 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/21:00548921 RIV/00216208:11310/21:10439304
Result on the web
<a href="https://doi.org/10.1126/science.abe2913" target="_blank" >https://doi.org/10.1126/science.abe2913</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/science.abe2913" target="_blank" >10.1126/science.abe2913</a>
Alternative languages
Result language
angličtina
Original language name
A ubiquitous disordered protein interaction module orchestrates transcription elongation
Original language description
During eukaryotic transcription elongation, RNA polymerase II (RNAP2) is regulated by a chorus of factors. Here, we identified a common binary interaction module consisting of TFIIS N-terminal domains (TNDs) and natively unstructured TND-interacting motifs (TIMs). This module was conserved among the elongation machinery and linked complexes including transcription factor TFIIS, Mediator, super elongation complex, elongin, IWS1, SPT6, PP1-PNUTS phosphatase, H3K36me3 readers, and other factors. Using nuclear magnetic resonance, live-cell microscopy, and mass spectrometry, we revealed the structural basis for these interactions and found that TND-TIM sequences were necessary and sufficient to induce strong and specific colocalization in the crowded nuclear environment. Disruption of a single TIM in IWS1 induced robust changes in gene expression and RNAP2 elongation dynamics, which underscores the functional importance of TND-TIM surfaces for transcription elongation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science
ISSN
0036-8075
e-ISSN
1095-9203
Volume of the periodical
374
Issue of the periodical within the volume
6571
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
1113-1121
UT code for WoS article
000725668600044
EID of the result in the Scopus database
2-s2.0-85120034108