Cavβ surface charged residues contribute to the regulation of neuronal calcium channels
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00551616" target="_blank" >RIV/61388963:_____/22:00551616 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/22:10436258 RIV/00216208:11120/22:43922757
Result on the web
<a href="https://doi.org/10.1186/s13041-021-00887-3" target="_blank" >https://doi.org/10.1186/s13041-021-00887-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13041-021-00887-3" target="_blank" >10.1186/s13041-021-00887-3</a>
Alternative languages
Result language
angličtina
Original language name
Cavβ surface charged residues contribute to the regulation of neuronal calcium channels
Original language description
Voltage-gated calcium channels are essential regulators of brain function where they support depolarization-induced calcium entry into neurons. They consist of a pore-forming subunit (Cavα1) that requires co-assembly with ancillary subunits to ensure proper functioning of the channel. Among these ancillary subunits, the Cavβ plays an essential role in regulating surface expression and gating of the channels. This regulation requires the direct binding of Cavβ onto Cavα1 and is mediated by the alpha interacting domain (AID) within the Cavα1 subunit and the α binding pocket (ABP) within the Cavβ subunit. However, additional interactions between Cavα1 and Cavβ have been proposed. In this study, we analyzed the importance of Cavβ3 surface charged residues in the regulation of Cav2.1 channels. Using alanine-scanning mutagenesis combined with electrophysiological recordings we identified several amino acids within the Cavβ3 subunit that contribute to the gating of the channel. These findings add to the notion that additional contacts besides the main AID/ABP interaction may occur to fine-tune the expression and properties of the channel.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Brain
ISSN
1756-6606
e-ISSN
1756-6606
Volume of the periodical
15
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
3
UT code for WoS article
000737986100002
EID of the result in the Scopus database
2-s2.0-85122180446