Organosulfur/selenium-based Highly Fluorogenic Molecular Probes for Live-Cell Nucleolus Imaging

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00556556" target="_blank" >RIV/61388963:_____/22:00556556 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/22:10445306

Result on the web

<a href="https://doi.org/10.1002/asia.202101281" target="_blank" >https://doi.org/10.1002/asia.202101281</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/asia.202101281" target="_blank" >10.1002/asia.202101281</a>

Result language

angličtina

Original language name

Organosulfur/selenium-based Highly Fluorogenic Molecular Probes for Live-Cell Nucleolus Imaging

Original language description

We present fluorogenic cationic organo chalcogens that are highly selective to RNA. We have demonstrated that the conformational dynamics and subsequently the optical properties of these dyes can be controlled to facilitate efficient bioimaging. We report the application of organoselenium and organosulfur-based cell-permeable red-emissive probes bearing a favorable cyclic sidearm for selective and high contrast imaging of cell nucleoli. The probes exhibit high quantum yield upon interacting with RNA in an aqueous solution. An in-depth multiscale simulation study reveals that the prominent rotational freezing of the electron-donating sidearm of the probes in the microenvironment of RNA helps in attaining more planar conformation when compared to DNA. It exerts a greater extent of intramolecular charge transfer and hence leads to enhanced fluorescence emission. A systematic structure-interaction relationship study highlighted the impact of heavy-chalcogens toward the improved emissive properties of the probes.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry-An Asian Journal

ISSN

1861-4728

e-ISSN

1861-471X

Volume of the periodical

17

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

e202101281

UT code for WoS article

000769575900001

EID of the result in the Scopus database

2-s2.0-85125380183