Non-enzymatic electrochemical determination of cholesterol in dairy products on boron-doped diamond electrode

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00558154" target="_blank" >RIV/61388963:_____/22:00558154 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10452228 RIV/61989592:15310/22:73615538
Result on the web
<a href="https://doi.org/10.1016/j.foodchem.2022.133278" target="_blank" >https://doi.org/10.1016/j.foodchem.2022.133278</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.foodchem.2022.133278" target="_blank" >10.1016/j.foodchem.2022.133278</a>

Result language
angličtina
Original language name
Non-enzymatic electrochemical determination of cholesterol in dairy products on boron-doped diamond electrode
Original language description
Determination of cholesterol in food matrices is essential for quality control concerning the health of consumers. Herein, a simple electrochemical approach for cholesterol quantitation in dairy products is evaluated. The newly developed differential pulse voltammetric method using acetonitrile-perchloric acid mixture as a supporting electrolyte is statistically compared to GC–MS and HPLC-UV. Oxidation signals of cholesterol at +1.5 V and +1.4 V (vs. Ag/AgNO3 in acetonitrile) provide detection limits of 4.9 µM and 6.1 µM on boron-doped diamond and glassy carbon electrodes, respectively. A simple liquid–liquid extraction procedure from dairy products into hexane resulted in a recovery rate of (74.8 ± 3.8)%. The method provides results in close agreement (at a 95% confidence level) with GC–MS, while HPLC-UV resulted in a significant difference in estimated cholesterol concentrations for all samples. This newly developed method is a simpler, faster and cheaper alternative to instrumentally demanding MS-based methods and clearly outperforms HPLC-UV.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
<a href="/en/project/GA19-11268S" target="_blank" >GA19-11268S: Electrochemical Methods: New Approaches for Characterization and Analysis of Steroids</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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