Traceless enzymatic synthesis of monodispersed hypermodified oligodeoxyribonucleotide polymers from RNA templates

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00561972" target="_blank" >RIV/61388963:_____/22:00561972 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/22:10451483

Result on the web

<a href="https://doi.org/10.1039/D2CC03588J" target="_blank" >https://doi.org/10.1039/D2CC03588J</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d2cc03588j" target="_blank" >10.1039/d2cc03588j</a>

Result language

angličtina

Original language name

Traceless enzymatic synthesis of monodispersed hypermodified oligodeoxyribonucleotide polymers from RNA templates

Original language description

We have developed a new alternative for enzymatic synthesis of single-stranded hypermodified oligodeoxyribonucleotides displaying four different hydrophobic groups based on reverse transcription from RNA templates catalyzed by DNA polymerases using a set of base-modified dNTPs followed by digestion of RNA by RNases. Using mixed oligodeoxyribonucleotide primers containing a ribonucleotide at the 3'-end, RNase AT1 simultaneously digested the template and cleaved off the primer to release a fully modified oligonucleotide that can be further 3'-labelled with a fluorescent nucleotide using TdT. The resulting hypermodified oligonucleotides could find applications in selection of aptamers or other functional macromolecules.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GX20-00885X" target="_blank" >GX20-00885X: Novel Functionalized (Bio)polymers Based on DNA Display of Small Molecules</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Communications

ISSN

1359-7345

e-ISSN

1364-548X

Volume of the periodical

58

Issue of the periodical within the volume

80

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

11248-11251

UT code for WoS article

000855389100001

EID of the result in the Scopus database

2-s2.0-85139378925