USP8 inhibition regulates autophagy flux and controls Salmonella infection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00571023" target="_blank" >RIV/61388963:_____/23:00571023 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/24:00559037

Result on the web

<a href="https://doi.org/10.3389/fcimb.2023.1070271" target="_blank" >https://doi.org/10.3389/fcimb.2023.1070271</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fcimb.2023.1070271" target="_blank" >10.3389/fcimb.2023.1070271</a>

Result language

angličtina

Original language name

USP8 inhibition regulates autophagy flux and controls Salmonella infection

Original language description

Introduction: Ubiquitination is an important protein modification that regulates various essential cellular processes, including the functions of innate immune cells. Deubiquitinases are enzymes responsible for removing ubiquitin modification from substrates, and the regulation of deubiquitinases in macrophages during infection with Salmonella Typhimurium and Yersinia enterocolitica remains unknown. Methods: To identify deubiquitinases regulated in human macrophages during bacterial infection, an activity-based proteomics screen was conducted. The effects of pharmacological inhibition of the identified deubiquitinase, USP8, were examined, including its impact on bacterial survival within macrophages and its role in autophagy regulation during Salmonella infection. Results: Several deubiquiitnases were differentially regulated in infected macrophages. One of the deubiquitinases identified was USP8, which was downregulated upon Salmonella infection. Inhibition of USP8 was associated with a decrease in bacterial survival within macrophages, and it was found to play a distinct role in regulating autophagy during Salmonella infection. The inhibition of USP8 led to the downregulation of the p62 autophagy adaptor. Discussion: The findings of this study suggest a novel role of USP8 in regulating autophagy flux, which restricts intracellular bacteria, particularly during Salmonella infection.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30107 - Medicinal chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Cellular and Infection Microbiology

ISSN

2235-2988

e-ISSN

2235-2988

Volume of the periodical

13

Issue of the periodical within the volume

March

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

1070271

UT code for WoS article

000962124300001

EID of the result in the Scopus database

2-s2.0-85151509577