Phosphorylation site of L-alanyl-L-glutamine identified by Raman optical activity spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00586928" target="_blank" >RIV/61388963:_____/24:00586928 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.saa.2024.124587" target="_blank" >https://doi.org/10.1016/j.saa.2024.124587</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.saa.2024.124587" target="_blank" >10.1016/j.saa.2024.124587</a>
Alternative languages
Result language
angličtina
Original language name
Phosphorylation site of L-alanyl-L-glutamine identified by Raman optical activity spectroscopy
Original language description
Phosphorylated peptides are instrumental in studying protein phosphorylation events. In the present study, Raman optical activity (ROA) is employed to elucidate the structure of a dipeptide, L-alanyl-L-glutamine (L-Ala-L-Gln) and its two differently alkylated N-phosphorylated derivatives. Theoretical simulations were conducted to aid the interpretation of peptide conformation variations upon phosphorylation, and of the measured Raman and ROA spectra. Induced circularly polarized luminescence (CPL) was also recorded in solution, in the presence of a simple europium aqua ion. As the spectra are peptide specific, this type of stereochemical analysis is expected to aid identification of the phosphorylation sites also in other peptides and possibly proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/GA23-05378S" target="_blank" >GA23-05378S: Synthesis and Characterization of Lanthanide-Luminescence Probes for Biomolecular Raman Spectroscopy and Imaging</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
ISSN
1386-1425
e-ISSN
1873-3557
Volume of the periodical
320
Issue of the periodical within the volume
November
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
124587
UT code for WoS article
001251417200001
EID of the result in the Scopus database
2-s2.0-85195218149